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Product Overview
| Product Name | AKR1C1 antibody |
|---|---|
| Catalog Number | GRP39 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.02 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q04828 |
| Entrez | 1645 |
Product Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GRP491 diluted at 1:1000
AKR1C1 antibody detects AKR1C1 protein by western blot analysis.A. 50 μg mouse liver lysate/extract10% SDS-PAGEAKR1C1 antibody (GRP491) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GRP491) diluted at 1:1000.
Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(GRP491) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(GRP491) antibody at 1:500 dilution.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GRP491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the
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