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Product Overview
| Product Name | ATR antibody [2B5] |
|---|---|
| Catalog Number | GRP84 |
| Species/Host | Mouse |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | FA, ICC, IF, IP, WB |
| Immunogen | Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: PBS |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Purity | Protein G purified |
| Clone ID | 2B5 |
| Uniprot ID | Q13535 |
| Entrez | 545 |
Product Description
Ataxia Telangiectasia Mutated (ATM) and Rad3-related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.
Application Notes
| Dilution Range | WB: 1:500-1:3000 |
|---|
Validation Images
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] (GRP536) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the](https://www.grp-ak.de/media/catalog/product/a/t/atr-antibody-2b5_grp536_wb_1_2.jpg)
Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GRP536) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunofluorescence analysis of HeLa, using ATR(GRP536) antibody at 1:100 dilution.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] (GRP536) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the
Detection of ATM (Anti-ATM 2C1, GRP536) or ATR (Anti-ATR 2B5) by western blot in human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
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