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Product Overview
| Product Name | AXL antibody |
|---|---|
| Catalog Number | GRP74 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ELISA, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AXL. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.63 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P30530 |
| Entrez | 558 |
Product Description
The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000,ELISA: 1:-1:10000 |
|---|
Validation Images
AXL antibody detects AXL protein by western blot analysis.A. 30 ?g 293T whole cell extractB. 30 ?g whole cell extract of human AXL-transfected 293T cells7.5 % SDS-PAGEAXL antibody (GRP526) dilution: 1:5000
AXL antibody detects AXL protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. AXL antibody (GRP526) dilution: 1:500.
AXL antibody detects AXL protein at cell membrane on human non-small cell lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human non-small cell lung carcinoma. AXL antibody (GRP526) diluted at 1:500.
An ELISA plate is coated with 50 ?L of AXL recombinant protein at concentration of 4 ?g/mL. The coated protein is detected with anti-AXL antibody (GRP526) at concentration ranged from 0.25 to 1000 ng/mL.
AXL antibody detects AXL protein at membrane on human non-small cell lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human non-small cell lung carcinoma. AXL antibody (GRP526) dilution: 1:500.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AXL antibody (GRP526) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AXL antibody (GRP526) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
AXL antibody detects AXL protein at cell membrane in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: AXL antibody (GRP526) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibod
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