Availability
- Request Lead Time
- In stock and ready for quick dispatch
- Usually dispatched within 5-10 working days
Product Overview
| Product Name | BDNF antibody |
|---|---|
| Catalog Number | GRP162 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BDNF. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.58 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P23560 |
| Entrez | 627 |
Product Description
The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BDNF antibody (GRP614) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the p
BDNF antibody detects BDNF protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. BDNF antibody (GRP614) diluted at 1:500.
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GRP614) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various tissue extracts (50 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GRP614) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat hippocampus.BDNF stained by BDNF antibody (GRP614) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat hippocampus.BDNF stained by BDNF antibody (GRP614) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.BDNF stained by BDNF antibody (GRP614) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.BDNF stained by BDNF antibody (GRP614) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.BDNF stained by BDNF antibody (GRP614) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.BDNF stained by BDNF antibody (GRP614) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BDNF antibody detects BDNF protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: BDNF protein stained by BDNF antibody (GRP614) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 an
BDNF antibody detects BDNF protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BDNF stained by BDNF antibody (GRP614) diluted at 1:500.Red: Tau, stained by Tau
Reviews
Write Your Own Review
