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beta Catenin antibody [N1N2-2], N-term

Catalog Number: GRP22

Product Overview

Product Name	beta Catenin antibody [N1N2-2], N-term
Catalog Number	GRP22
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat, Rabbit
Conjugation	Unconjugated
Tested applications	ChIP, FACS, ICC, IF, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.
Alternative Names	(click to expand) catenin beta 1 \| CTNNB \| EVR7 \| MRD19 \| armadillo

Product Properties

Form/Appearance	Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P35222
Entrez	1499

Product Description

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

Application Notes

Dilution Range	WB: 1:500-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,FACS: 1:50-1:200,IP: 1:50-1:100

Validation Images

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:500.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ?g of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. beta Catenin antibody [N1N2-2], N-term (GRP474) dilution: 1:500.

beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by western blot analysis.A. 30 μg PC-12 whole cell lysate/extract

7.5% SDS-PAGEbeta Catenin antibody [N1N2-2], N-term (GRP474) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [N1N2-

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:50

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:500.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. beta Catenin antibody [N1N2-2], N-term (GRP474) dilution: 1:500.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:500.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2],

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:500.

Sample (50 μg of whole cell lysate) A: mouse brain 7.5% SDS PAGE GRP474 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin(GRP474) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:10000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GRP474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibo

beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta Catenin antibody [N1N2-2] (GRP474) diluted at 1:500.Red: alpha Tubulin antibody

Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 ?g of beta Catenin antibody (GRP474).Western blot analysis was performed using beta Catenin antibody (GRP474).EasyBlot anti-Rabbit IgG was used as a secondary reagent.

beta Catenin antibody [N1N2-2], N-term (GRP474) detects CTNNB1 protein by flow cytometry analysis. Sample: HeLa cell. Black: Unlabelled sample was used as a control. Red: beta Catenin antibody [N1N2-2], N-term (GRP474) dilution: 1:50. Acquisition o

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