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beta Tubulin 3/ Tuj1 antibody [GT11710]
Catalog Number: GRP174
Product Overview
Product Name | beta Tubulin 3/ Tuj1 antibody [GT11710] |
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Catalog Number | GRP174 |
Species/Host | Mouse |
Reactivity | Human, Mouse, Rat |
Conjugation | Unconjugated |
Tested applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human beta Tubulin 3/ Tuj1. The exact sequence is proprietary. |
Alternative Names | (click to expand) |
Product Properties
Form/Appearance | Liquid: PBS |
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Concentration | 1 mg/ml |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
Note | For research use only. |
Isotype | IgG2b |
Clonality | Monoclonal |
Purity | Affinity purified by Protein A. |
Clone ID | GT11710 |
Uniprot ID | Q13509 |
Entrez | 10381 |
Product Description
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]
Application Notes
Dilution Range | WB: 1:500-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,IP: 1:100-1:500 |
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Validation Images
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.Sample: Frozen sectioned E13.5 rat brain. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GRP626) diluted at
beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis. Sample:Frozen section of embryonic mouse brain (mE18.5). Red: beta III Tubulin antibody [GT11710] (GRP626) diluted at 1:500. Bl
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GRP626) diluted
Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GRP626) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Tubulin 3/ TUJ1
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. beta Tubulin 3/ TUJ1 antibody [GT11710] (GRP626) diluted at 1:500.
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GRP626) diluted at a dilut
Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GRP626) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.Sample: Frozen sectioned E13.5 rat brain.Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP626) diluted at 1:250.Red: beta Tubulin 3/ TUJ1 p
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