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Product Overview
| Product Name | Caspase 3 antibody |
|---|---|
| Catalog Number | GRP46 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.61 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P42574 |
| Entrez | 836 |
Product Description
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Caspase 3 antibody detects Caspase 3 protein by western blot analysis.A. 30 μg C8D30 whole cell extractB. 30 μg NIH-3T3 whole cell extractC. 30 μg Raw264.7 whole cell extractD. 30 μg C2C12 whole cell extract12% SDS-PAGECaspase 3 antibody (GRP4
Caspase 3 antibody detects Caspase 3 protein by western blot analysis.A. 30 μg Jurkat whole cell lysate/extractB. 30 μg Raji whole cell lysate/extractC. 30 μg NCI-H929 whole cell lysate/extract 12% SDS-PAGECaspase 3 antibody (GRP498) dilution: 1:
Caspase 3 antibody detects CASP3 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CASP3 protein stained by Caspase 3 antibody (GRP498) diluted at 1:500.Blue: Hoechst 33343 sta
Caspase 3 antibody detects Caspase 3 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Caspase 3 antibody (GRP498) diluted at 1:500.
Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3
Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GRP498) diluted by 1:1000. The HRP-conjugated anti-rabbit I
Caspase 3 antibody detects Caspase 3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.Caspase 3 stained by Caspase 3 antibody (GRP498) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The WB analysis of Caspase 3 antibody was published by Zera K and colleagues in the journal PLoS One in 2017.PMID: 29045486
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GRP498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primar
Immunoprecipitation of Caspase 3 protein from HeLa whole cell extracts using 5 ?g of Caspase 3 antibody (GRP498).Western blot analysis was performed using Caspase 3 antibody (GRP498).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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