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Product Overview
| Product Name | CD71 antibody [N2C1], Internal |
|---|---|
| Catalog Number | GRP27 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD71. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P02786 |
| Entrez | 7037 |
Product Description
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000 |
|---|
Validation Images
![CD71 antibody [N2C1], Internal detects CD71 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD71 protein stained by CD71 antibody [N2C1], Internal (GRP479) diluted at 1:500.Bl](https://www.grp-ak.de/media/catalog/product/c/d/cd71-antibody-n2c1-internal_grp479_if_1_2.jpg)
CD71 antibody detects CD71 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody (GRP479) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit I
CD71 antibody [N2C1], Internal detects CD71 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD71 protein stained by CD71 antibody [N2C1], Internal (GRP479) diluted at 1:500.Bl
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