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Product Overview
Product Name | Collagen III antibody [C2C3], C-term |
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Catalog Number | GRP32 |
Species/Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Conjugation | Unconjugated |
Tested applications | IHC-P, WB |
Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III. The exact sequence is proprietary. |
Alternative Names | (click to expand) |
Product Properties
Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
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Concentration | 0.46 mg/ml |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
Note | For research use only. |
Isotype | IgG |
Clonality | Polyclonal |
Purity | Purified by antigen-affinity chromatography. |
Uniprot ID | P02461 |
Entrez | 1281 |
Product Description
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
Application Notes
Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000 |
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Validation Images
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.A. 50 μg rat liver lysate/extract 5% SDS-PAGECOL3A1 antibody [C2C3], C-term (GRP484) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to de
Immunohistochemical analysis of paraffin-embedded human PAPILLARY CA_STROMAL CELLS, using Collagen III (GRP484) antibody at 1:250 dilution.
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.A. 30 μg Neuro2A whole lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract D. 30 μg Raw264.7 whole cell l
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. Mouse tissue extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GRP484) at a dilution of 1:1
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.A. 30 μg PC-12 whole cell extract B. 30 μg Rat2 whole cell extract5% SDS-PAGECollagen III antibody [C2C3], C-term (GRP484) dilution: 1:1000 The HRP-conjugated
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