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Donkey anti-Rabbit IgG (H&L), DyLight® 488 conjugated, min. cross-reactivity to human IgG and serum proteins

Catalog Number: GRP12320

Product Overview
Product Name Donkey anti-Rabbit IgG (H&L), DyLight® 488 conjugated, min. cross-reactivity to human IgG and serum proteins
Catalog Number GRP12320
Species/Host Donkey
Immunogen Purified Rabbit IgG, whole molecule
Product Properties
Form/Appearance Lyophilized
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note For research use only.
Clonality Polyclonal
Purity Affinity purified donkey IgG
Application Notes
Dilution Range 1 : 20-1 : 2000 for most applications
Application Notes Additional Information: Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins, mouse IgG or serum proteins Background: Donkey anti-Rabbit IgG (H&L), DyLight®488 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight® 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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