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Fibronectin antibody [N1N2], N-term

Catalog Number: GRP53

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Datasheet
Product Overview
Product Name Fibronectin antibody [N1N2], N-term
Catalog Number GRP53
Species/Host Rabbit
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Tested applications ELISA, ICC, IF, IHC-Fr, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.
Alternative Names (click to expand) fibronectin 1 | CIG | ED-B | FINC | FN | FNZ | GFND | GFND2 | LETS | MSF | SMDCF
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.14 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P02751
Entrez 2335
Product Description

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500,ELISA: 1:1000-1:10000
Validation Images
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin (GRP505) antibody at 1:200 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin (GRP505) antibody at 1:200 dilution.
Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term (GRP505) dilution: 1:500.
Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term (GRP505) dilution: 1:500.
Fibronectin antibody detects Fibronectin protein by Western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody (GRP505) at a dilution of 1:1000.
Fibronectin antibody detects Fibronectin protein by Western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody (GRP505) at a dilution of 1:1000.
Indirect ELISA analysis was performed by coating plate with 100 μL of recombinant Fibronectin protein at concentration of 10 μg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GRP505) at rangeing from 0.5 to 140 ng/mL.
Indirect ELISA analysis was performed by coating plate with 100 μL of recombinant Fibronectin protein at concentration of 10 μg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GRP505) at rangeing from 0.5 to 140 ng/mL.
Competitive ELISA detection of human fibronectin in plasma using GRP505 diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1:2,000 and used to detect
Competitive ELISA detection of human fibronectin in plasma using GRP505 diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1:2,000 and used to detect
Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GRP505) diluted at 1:1000. The HRP-conjugated a
Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GRP505) diluted at 1:1000. The HRP-conjugated a
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GRP505) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GRP505) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to
The WB analysis of Fibronectin antibody [N1N2], N-term was published by Pon JR and colleagues in the journal Nat Commun in 2015.PMID: 26245647
The WB analysis of Fibronectin antibody [N1N2], N-term was published by Pon JR and colleagues in the journal Nat Commun in 2015.PMID: 26245647
The WB analysis of Fibronectin antibody [N1N2], N-term was published by Pon JR and colleagues in the journal Nat Commun in 2015.PMID: 26245647
The WB analysis of Fibronectin antibody [N1N2], N-term was published by Pon JR and colleagues in the journal Nat Commun in 2015.PMID: 26245647
Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ?g whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 ?g of pre-immune ra
Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ?g whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 ?g of pre-immune ra
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