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Product Overview
| Product Name | GAD67 antibody |
|---|---|
| Catalog Number | GRP108 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.4 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q99259 |
| Entrez | 2571 |
Product Description
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
GAD67 antibody detects GAD67 protein by western blot analysis.A. 30 μg 293T whole cell lysate/extractB. 30 μg whole cell lysate/extract of human GAD1-transfected 293T cells7.5% SDS-PAGEGAD67 antibody (GRP560) dilution: 1:5000The HRP-conjugated anti-
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GRP560) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GRP560) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GRP560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GRP560) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.GAD67 stained by GAD67 antibody (GRP560) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.GAD67 stained by GAD67 antibody (GRP560) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.GAD67 stained by GAD67 antibody (GRP560) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GAD67 stained by GAD67 antibody (GRP560) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAD67 antibody detects GAD67 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD67 stained by GAD67 antibody (GRP560) diluted at 1:500.Red: Tau, stained by
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