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Goat anti-Chicken IgY (H&L), DyLight® 405 conjugated
Catalog Number: GRP12999
Product Overview
| Product Name | Goat anti-Chicken IgY (H&L), DyLight® 405 conjugated |
|---|---|
| Catalog Number | GRP12999 |
| Species/Host | Goat |
| Reactivity | Chicken |
| Tested applications | FC, IL |
| Immunogen | Purified Chicken IgY, whole molecule. |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store freeze-dried powder at 2-8 °C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified |
Application Notes
| Dilution Range | 1 : 20-1 : 2000 (Flow cyt), (IL) |
|---|---|
| Application Notes | Additional Information: Antibody purity is > 95% based on SDS-PAGE.DyLight® 405 (Ex = 400 nm; Em = 420 nm)Affinity purified using solid phase Chicken IgG/Y.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: • heavy (γ) chains on chicken IgG/Y • light chains on all chicken immunoglobulins .Based on IEP, no reactivity is observed to: • non-immunoglobulin chicken serum immunoglobulins . Background: Goat anti-Chicken IgY (H&L) is a secondary antibody, conjugated to DyLight® 405, which binds to chicken IgY (H&L) in immunological assays.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. Reconstitution: For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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