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Goat anti-Rat IgG (H&L), ALP conjugated, min. reactivity to Human and mouse IgG, highly adsorbed against mouse IgG
Catalog Number: GRP12612
Product Overview
Product Name | Goat anti-Rat IgG (H&L), ALP conjugated, min. reactivity to Human and mouse IgG, highly adsorbed against mouse IgG |
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Catalog Number | GRP12612 |
Species/Host | Goat |
Reactivity | Rat |
Predicted Reactivity | Heavy chains on Rat IgG and with the Light chains on all Rat immunoglobulins based on IEP. |
Tested applications | ELISA, ICC, WB, IHC |
Immunogen | Purified Rat IgG, whole molecule |
Product Properties
Form/Appearance | Liquid |
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Storage | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
Note | For research use only. |
Clonality | Polyclonal |
Purity | Purified goat IgG |
Application Notes
Dilution Range | 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) |
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Application Notes | Additional Information: This antibody is highly cross absorbed against mouse IgG. No reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis. No reactivity is observed to human or mouse IgG based on immunoelecrophoresis. APL conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative Background: Goat anti-rat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays. |
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