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Product Overview
Product Name | GRK2 antibody [C2C3], C-term |
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Catalog Number | GRP107 |
Species/Host | Rabbit |
Reactivity | Human, Mouse |
Conjugation | Unconjugated |
Tested applications | FACS, ICC, IF, IHC-P, IP, WB |
Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary. |
Alternative Names | (click to expand) |
Product Properties
Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
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Concentration | 1.04 mg/ml |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
Note | For research use only. |
Isotype | IgG |
Clonality | Polyclonal |
Purity | Purified by antigen-affinity chromatography. |
Uniprot ID | P25098 |
Entrez | 156 |
Product Description
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]
Application Notes
Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
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Validation Images
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GRP559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GRK2(GRP559) antibody at 1:500 dilution.
Sample (30 μg of whole cell lysate) A:NIH-3T37.5% SDS PAGE GRP559 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GRK2(GRP559) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GRP559) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample(30 μg whole cell lysate)A:MOLT4 (GRP559)7.5% SDS PAGE diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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