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Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]

Catalog Number: GRP79

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Datasheet
Product Overview
Product Name Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]
Catalog Number GRP79
Species/Host Mouse
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, IP, WB
Immunogen Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary.
Alternative Names (click to expand) H2A histone family member X | H2A.X | H2A/X | H2AX
Product Properties
Form/Appearance Liquid: PBS
Concentration 0.92 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG1
Clonality Monoclonal
Purity Affinity purified by Protein G.
Clone ID GT2311
Uniprot ID P16104
Entrez 3014
Product Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) antibody [
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) antibody [
Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10?M Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibod
Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10?M Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibod
Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.A. 30 μg NIH-3T3 whole cell lysate/extract (untreated)B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr)15% SDS-PAGEHistone
Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.A. 30 μg NIH-3T3 whole cell lysate/extract (untreated)B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr)15% SDS-PAGEHistone
Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 1
Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 1
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and t
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and t
Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.A. 30 μg HCT116 whole cell lysate/extract (untreated)B. 30 μg HCT116 whole cell lysate/extract (30 μM cisplatin treatment for 24hr)12% SDS-PAGEHistone H
Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.A. 30 μg HCT116 whole cell lysate/extract (untreated)B. 30 μg HCT116 whole cell lysate/extract (30 μM cisplatin treatment for 24hr)12% SDS-PAGEHistone H
The WB analysis of Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] was published by En J and colleagues in the journal PLoS Negl Trop Dis in 2017.PMID: 28783752
The WB analysis of Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] was published by En J and colleagues in the journal PLoS Negl Trop Dis in 2017.PMID: 28783752
Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 ?g HCT116 with CPT 30 ?M treatment 24 hr whole cell lysate/extract A. 30 ?g HCT116 whole with CPT 30 uM treatment cell lysate/e
Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 ?g HCT116 with CPT 30 ?M treatment 24 hr whole cell lysate/extract A. 30 ?g HCT116 whole with CPT 30 uM treatment cell lysate/e
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10?M Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: H2A.X protein stained
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10?M Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: H2A.X protein stained
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