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Product Overview
| Product Name | Iba1 antibody |
|---|---|
| Catalog Number | GRP93 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | FACS, ICC, IF, IHC-Fr, IHC-P, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.14 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P55008 |
| Entrez | 199 |
Product Description
This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,FACS: 1:50-1:200 |
|---|
Validation Images
Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Iba1 antibody (GRP545) dilution: 1:500.
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GRP545) diluted at 1:1000.
Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody (GRP545) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker,
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1(GRP545) antibody at 1:500 dilution.
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GRP545) diluted at 1:1000.
Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Iba1 antibody (GRP545) dilution: 1:500.
Iba1 antibody (GRP545) detects AIF1 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: Iba1 antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody (GRP545) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Iba1 stained by Iba1 antibody (GRP545) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GRP545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Iba1 antibody detects Iba1 protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody (GRP545) diluted at 1:500.Blue: Hoechst 33342 staining.
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Iba1 stained by Iba1 antibody (GRP545) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Iba1 stained by Iba1 antibody (GRP545) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The ICC/IF analysis of Iba1 antibody was published by Wolf H and colleagues in the journal Dis Model Mech in 2016 .
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