KLH - Keyhole limpet hemocyanin, ALP-conjugated (40 µg)

Catalog Number: GRP13173

Product Overview

Product Name	KLH - Keyhole limpet hemocyanin, ALP-conjugated (40 µg)
Catalog Number	GRP13173
Species/Host	Rabbit
Reactivity	Limpet
Tested applications	ELISA
Immunogen	Purified keyhole limpet hemocyanin (KLH), whole molecule,

Product Properties

Form/Appearance	Liquid, conjugated to ALP
Storage	Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Note	For research use only.
Clonality	Polyclonal
Purity	Affinity purified serum in PBS, pH 7.4
MW	ca. 400 kDa/subunit
Uniprot ID	Q6KC56

Application Notes

Dilution Range	1 : 5 000 (ELISA), 1 : 1000 (IL), 1 : 5 000 (WB)
Application Notes	Additional Information: Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.Optimal working dilution has to be determined by end user. Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide. Further information about it can be found here. Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Dilution Range

1 : 5 000 (ELISA), 1 : 1000 (IL), 1 : 5 000 (WB)

Application Notes

Additional Information: Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.Optimal working dilution has to be determined by end user. Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide. Further information about it can be found here. Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

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