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KLH - Keyhole limpet hemocyanin, HRP-conjugated (40 µg)
Catalog Number: GRP13174
Product Overview
| Product Name | KLH - Keyhole limpet hemocyanin, HRP-conjugated (40 µg) |
|---|---|
| Catalog Number | GRP13174 |
| Species/Host | Rabbit |
| Reactivity | Limpet |
| Tested applications | ELISA, WB, IL |
| Immunogen | Purified keyhole limpet hemocyanin (KLH), whole molecule, |
Product Properties
| Form/Appearance | Liquid |
|---|---|
| Storage | Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified serum in PBS, pH 7.4 |
| MW | ca. 400 kDa/subunit |
| Uniprot ID | Q6KC56 |
Application Notes
| Dilution Range | 1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL) |
|---|---|
| Application Notes | Additional Information: Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE. Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide. Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy. |
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