MMP2 antibody

Product Overview

Product Name	MMP2 antibody
Catalog Number	GRP34
Species/Host	Rabbit
Reactivity	Human, Mouse
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human MMP2. The exact sequence is proprietary.
Alternative Names	(click to expand) matrix metallopeptidase 2 \| CLG4 \| CLG4A \| MMP-2 \| MMP-II \| MONA \| TBE-1

Product Properties

Form/Appearance	Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.37 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P08253
Entrez	4313

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500

Validation Images

Immunofluorescence analysis of methanol-fixed HeLa, using MMP2(GRP486) antibody at 1:200 dilution.

MMP2 antibody detects MMP2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MMP2 antibody (GRP486) dilution: 1:500.

Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GRP486 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

MMP2 antibody detects MMP2 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. MMP2 antibody (GRP486) dilution: 1:500.

MMP2 antibody detects MMP2 protein at extracellular matrix in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. MMP2 antibody (GRP486) diluted at 1:500.

MMP2 antibody detects MMP2 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. MMP2 antibody (GRP486) dilution: 1:500.

MMP2 antibody detects MMP2 protein at extracellular matrix in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. MMP2 antibody (GRP486) diluted at 1:500.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GRP486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GRP486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

The WB analysis of MMP2 antibody was published by Gao Y and colleagues in the journal Sci Rep in 2016.PMID: 26813495

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GRP486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (GRP486) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunoprecipitation of MMP2 protein from U87-MG whole cell extracts using 5 ?g of MMP2 antibody (GRP486).Western blot analysis was performed using MMP2 antibody (GRP486).EasyBlot anti-Rabbit IgG was used as a secondary reagent.

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MMP2 antibody

Catalog Number: GRP34