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mTOR antibody [C3], C-term

Catalog Number: GRP24

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Datasheet
Product Overview
Product Name mTOR antibody [C3], C-term
Catalog Number GRP24
Species/Host Rabbit
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Tested applications ChIP, ICC, IF, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.
Alternative Names (click to expand) mechanistic target of rapamycin kinase | FRAP | FRAP1 | FRAP2 | RAFT1 | RAPT1 | SKS
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.27 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P42345
Entrez 2475
Product Description

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. mTOR antibody (GRP476) dilution: 1:500.
mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. mTOR antibody (GRP476) dilution: 1:500.
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. mTOR antibody [C3], C-term (GRP476) diluted at 1:500.
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. mTOR antibody [C3], C-term (GRP476) diluted at 1:500.
mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GRP476) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to
mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GRP476) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to
Cross-linked ChIP was performed with 293T chromatin extract and 5 ?g of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .
Cross-linked ChIP was performed with 293T chromatin extract and 5 ?g of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .
mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. mTOR antibody [C3], C-term (GRP476) diluted at 1:500.
mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. mTOR antibody [C3], C-term (GRP476) diluted at 1:500.
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: mTOR stained by mTOR antibody [C3], C-term (GRP476) diluted at 1:2000.Blue: Hoechst 33342 staining.
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: mTOR stained by mTOR antibody [C3], C-term (GRP476) diluted at 1:2000.Blue: Hoechst 33342 staining.
mTOR antibody detects mTOR protein by Western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GRP476) diluted at a dilution of 1:1000.
mTOR antibody detects mTOR protein by Western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GRP476) diluted at a dilution of 1:1000.
The WB analysis of mTOR antibody [C3], C-term was published by Chen HR and colleagues in the journal Biol Open in 2015.PMID: 25617421
The WB analysis of mTOR antibody [C3], C-term was published by Chen HR and colleagues in the journal Biol Open in 2015.PMID: 25617421
mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 ?g of pre-immune rabbit IgG B : Immunoprecipitation of mTOR by 3 ?g of mTOR antibody (GRP476) 5% SDS-PAGE The immunoprecipitated
mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 ?g of pre-immune rabbit IgG B : Immunoprecipitation of mTOR by 3 ?g of mTOR antibody (GRP476) 5% SDS-PAGE The immunoprecipitated
Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 ?g of mTOR antibody [C3], C-term (GRP476).Western blot analysis was performed using mTOR antibody [C3], C-term (GRP476).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 ?g of mTOR antibody [C3], C-term (GRP476).Western blot analysis was performed using mTOR antibody [C3], C-term (GRP476).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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