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Product Overview
| Product Name | OLIG2 antibody |
|---|---|
| Catalog Number | GRP163 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Pig |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human OLIG2. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.82 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q13516 |
| Entrez | 10215 |
Product Description
This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000 |
|---|
Validation Images
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with OLIG2 antibody (GRP615) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with OLIG2 antibody (GRP615) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with OLIG2 antibody (GRP615) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
OLIG2 antibody detects OLIG2 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: OLIG2 protein stained by OLIG2 antibody (GRP615) diluted at 1:500.Red
OLIG2 antibody detects OLIG2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: OLIG2 stained by OLIG2 antibody (GRP615) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta
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