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PARP antibody [N2C1], Internal

Catalog Number: GRP54

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Datasheet
Product Overview
Product Name PARP antibody [N2C1], Internal
Catalog Number GRP54
Species/Host Rabbit
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Tested applications ChIP, ICC, IF, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.
Alternative Names (click to expand) poly(ADP-ribose) polymerase 1 | ADPRT | ADPRT 1 | ADPRT1 | ARTD1 | PARP | PARP-1 | PPOL | pADPRT-1
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.23 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P09874
Entrez 142
Product Description

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Application Notes
Dilution Range WB: 1:500-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
Cross-linked ChIP was performed with Raji chromatin extract and 5 ?g of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
Cross-linked ChIP was performed with Raji chromatin extract and 5 ?g of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PARP1 antibody [N2C1], Internal (GRP506) dilution: 1:500.
PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PARP1 antibody [N2C1], Internal (GRP506) dilution: 1:500.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GRP506) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GRP506) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.
ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GRP506) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was u
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GRP506) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was u
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GRP506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GRP506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GRP506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to de
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GRP506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to de
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GRP506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GRP506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PARP stained by PARP antibody [N2C1], Internal (GRP506) diluted at 1:500.Red: phalloi
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PARP stained by PARP antibody [N2C1], Internal (GRP506) diluted at 1:500.Red: phalloi
PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments.IP samples: HCT-116 whole cell extractA. 30 ?g HCT-116 whole cell extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of PARP1 protein by 4 ?g PARP1 a
PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments.IP samples: HCT-116 whole cell extractA. 30 ?g HCT-116 whole cell extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of PARP1 protein by 4 ?g PARP1 a
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