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SARS-CoV-2 trimeric spike protein (D614G)
Catalog Number: GRP10348
Product Overview
| Product Name | SARS-CoV-2 trimeric spike protein (D614G) |
|---|---|
| Catalog Number | GRP10348 |
Product Properties
| Concentration | 1 mg/ml |
|---|---|
| Storage | -20 |
| Note | For research use only. |
Product Description
| Mopdification | C-terminal transmembrane region replaced with a trimerization domain and a polyhistidine tag. Two stabilizing proline mutations and scrambled S1/S2 furin cleavage site (see reference). D614G mutation. |
| Isolate (Seq ID) | Wuhan-Hu-1 (GenBank: MN908947), D614G mutation |
| Expression System | CHOExpressTM cells |
| Purity | > 90 % as determined by SDS-PAGE |
| Conjugate | His |
| N-terminus | VNLT |
| Molecular Weight | The recombinant SARS-CoV-2 trimeric spike protein consists of 3576 amino acids with a predicted molecular mass of ~400 kDa |
| Endotoxin | <1.0 EU per μg protein as determined by the LAL method |
| Format | Liquid |
| Concentration | 1.0 mg/ml |
| Buffer | 0.01M PBS, pH 7.4 |
| Preservative | None |
| Storage | Store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. |
| Ship | Shipped on dry ice |
References
Wrapp D. et al. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science 367, 1260–1263 (2020)
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