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Product Overview
| Product Name | Tau antibody |
|---|---|
| Catalog Number | GRP99 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Tau. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.34 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P10636 |
| Entrez | 4137 |
Product Description
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody (GRP551) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody (GRP551) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Tau stained by Tau antibody (GRP551) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Tau stained by Tau antibody (GRP551) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj
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