TET1 antibody [GT1462]

Catalog Number: GRP78

Product Overview

Product Name	TET1 antibody [GT1462]
Catalog Number	GRP78
Species/Host	Mouse
Reactivity	Human, Mouse
Conjugation	Unconjugated
Tested applications	ChIP, ICC, IF, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.
Alternative Names	(click to expand) tet methylcytosine dioxygenase 1 \| CXXC6 \| LCX \| bA119F7.1

Product Properties

Form/Appearance	Liquid: PBS
Concentration	1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG2a
Clonality	Monoclonal
Purity	Affinity purified by Protein G.
Clone ID	GT1462
Uniprot ID	Q8NFU7
Entrez	80312

Product Description

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500

Validation Images

TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 antibody [GT1462] (GRP530) dilution: 1:100.

TET1 antibody [GT1462] detects TET1 protein by western blot analysis.A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells6% SDS-PAGETET1 antibody [GT14

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GRP530

NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GRP530) diluted at 1:500.

TET1 antibody detects TET1 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells5 % SDS-PAGETET1 antibody (GRP530) dilution: 1:500

Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 ?g of TET1 antibody [GT1462] (GRP530).Western blot analysis was performed using TET1 antibody [GT1462] (GRP530) diluted at 1:500.EasyBlot anti-Mouse IgG was used as a secondary rea

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