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Product Overview
| Product Name | Twist1/2 antibody |
|---|---|
| Catalog Number | GRP66 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Drosophila |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Twist1/2. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
Product Description
This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]
Application Notes
| Dilution Range | WB: 1:500-1:10000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Twist1/2 antibody (GRP518) dilution: 1:500.
Twist1/2 antibody detects Twist1/2 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Twist1/2 antibody (GRP518) dilution: 1:500.
Twist1/2 antibody detects Twist1/2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GRP518) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG
Twist1/2 antibody detects Twist1 protein by western blot analysis. Non-transfected (-) and Twist1-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GRP518) diluted at 1
Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Twist1/2 antibody (GRP518) dilution: 1:500.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GRP518) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The WB analysis of Twist1/2 antibody was published by Ju SY and colleagues in the journal Cell Death Dis in 2015.PMID: 26136074
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